In the wee hours of Friday, Trinamul Congress Birbhum district president Anubrata Mondal reached the CBI office Nizam Palace in Calcutta from Asansol Special Court.
CBI has been given 10-day custody of Mondal in the cattle smuggling case.
The party strongman was arrested by CBI in a cattle smuggling case on Thursday, three weeks after Enforcement Directorate picked up another heavyweight leader Partha Chatterjee in the school recruitment scam.
Mondal, who was arrested from his residence in Bolpur in Birbhum district after an hour-long interrogation, was sent to CBI custody till August 20 by a special CBI court.
The ruling party of West Bengal decided to hit the streets for two consecutive days against the vendetta politics allegedly being pursued by the BJP-led central government by using its agencies.
Opposition BJP alleged that Mondal's arrest proves that TMC is neck-deep in corruption and demanded a statement from Chief Minister Mamata Banerjee on this.
Mondal whose political career was marked by controversies skipped his scheduled appearance before the central probe agency on Monday and Wednesday, citing ill health.
"We have arrested him for non-cooperation in the investigation into the cattle smuggling case. We have found Mr Mondal's direct involvement in the scam, a CBI official told PTI.
(With PTI inputs)